ASSIGNMENT OF THE GENE ENCODING THE CATALYTIC SUBUNIT-C-BETA OF CAMP-DEPENDENT PROTEIN-KINASE TO THE P36-BAND ON CHROMOSOME-1

A cDNA for the human catalytic subunit (C-beta) of cAMP-dependent prot ein kinase (PKA) has been cloned from a testis cDNA library. In the pr esent study, we have determined the chromosomal localization of this g ene using a cDNA for C-beta as a probe. Southern blot analysis of geno mic DNA from human/mouse cell hybrids revealed that the presence or ab sence of a 20-kb XbaI fragment, which hybridized with the C-beta probe , was concordant with the presence of human chromosome 1. In situ hybr idization to metaphase chromosome confirmed the somatic cell hybrid da ta and regionally mapped the C-beta gene of PKA to the p36 band on chr omosome 1.