2 INTRAGENIC POLYMORPHISMS OF THE APC-GENE DETECTED BY PCR AND ENZYMATIC DIGESTION

Authors
KRAUS C BALLHAUSEN WG
Citation
C. Kraus et Wg. Ballhausen, 2 INTRAGENIC POLYMORPHISMS OF THE APC-GENE DETECTED BY PCR AND ENZYMATIC DIGESTION, Human genetics, 88(6), 1992, pp. 705-706
Citations number
2
Journal title
Human genetics → ACNP
ISSN journal
03406717
Volume
88
Issue
6
Year of publication
1992
Pages
705 - 706
Database
ISI
SICI code
0340-6717(1992)88:6<705:2IPOTA>2.0.ZU;2-8
Abstract
The gene causing adenomatous polyposis coli (APC) has recently been cl oned. Three intragenic polymorphism were reported to be detectable by single-strand conformation polymorphism analysis. Here, we describe an assay using polymerase-chain-reaction-based amplification and subsequ ent enzymatic digestion of genomic sequences to identify polymorphic s ites at nucleotide positions 1458 and 5037 of the APC gene.