THE INVOLVEMENT OF ACTIVE DNA-SYNTHESIS IN CAMPTOTHECIN-INDUCED G2 ARREST - ALTERED REGULATION OF P34CDC2 CYCLIN-B

Cell cycle arrest in G2 phase is a common response to a variety of DNA -damaging agents. The coupling between DNA damage and G2 arrest was st udied in synchronized HeLa cells using camptothecin, a highly specific inhibitor of topoisomerase I that damages DNA through the formation o f reversible topoisomerase I-DNA cleavable complexes. Brief camptothec in treatment of early S-phase HeLa cells caused arrest at G2 phase and abolished the activation of p34cdc2 protein kinase. Both tyrosine dep hosphorylation of p34cdc2 and cyclin B accumulation were altered. Thes e cell cycle-dependent changes were not observed when DNA replication was inhibited by aphidicolin during the brief camptothecin treatment. Our results suggest that to produce G2 arrest, active DNA synthesis is required at the time of camptothecin treatment, as was previously sho wn for camptothecin-induced cytotoxicity. Furthermore, our results sug gest that the interaction of the replication fork with DNA damage may ultimately trigger altered regulation of p34cdc2/cyclin B, leading to cell cycle arrest at the G2 phase.