CYTOSOLIC FREE CALCIUM INCREASED BY PROSTAGLANDIN-F2-ALPHA (PGF2-ALPHA), GONADOTROPIN-RELEASING-HORMONE, AND ANGIOTENSIN-II IN RAT GRANULOSA-CELLS AND PGF2-ALPHA IN HUMAN GRANULOSA-CELLS
Wd. Currie et al., CYTOSOLIC FREE CALCIUM INCREASED BY PROSTAGLANDIN-F2-ALPHA (PGF2-ALPHA), GONADOTROPIN-RELEASING-HORMONE, AND ANGIOTENSIN-II IN RAT GRANULOSA-CELLS AND PGF2-ALPHA IN HUMAN GRANULOSA-CELLS, Endocrinology, 130(4), 1992, pp. 1837-1843
Cytosolic [Ca2+]i was measured using a microspectrofluorimetric techni
que. Prostaglandin F2-alpha (PGF2-alpha, 10(-6) M) transiently increas
ed the concentration of free cytosolic Ca2+ ([Ca2+]i) in individual ra
t and human granulosa cells. In a study examining a total of 170 indiv
idual rat and human granulosa cells, approximately 100% of rat granulo
sa cells and 80% of human granulosa cells tested responded to PGF2-alp
ha (10(-6) M). In a dose-response trial, the magnitude of the [Ca2+]i
response did not vary, although a decreasing number of cells responded
to decreasing PGF2-alpha concentrations (10(-5) to 10(-9) M). PGE2 (1
0(-4) to 10(-6) M) did not affect [Ca2+]i in rat or human granulosa ce
lls. GnRH (10(-6) M) increased [Ca2+]i in rat but not human granulosa
cells. Over 90% of rat granulosa cells tested responded. Angiotensin I
I (ANG II, 10(-5) M) increased [Ca2+]i in approximately 25% of rat, bu
t not human granulosa cells. Individual rat granulosa cells which resp
onded to GnRH responded to PGF2-alpha and vice versa. Individual rat g
ranulosa cells which responded to ANG II responded to PGF2-alpha and G
nRH. Conversely, less than 30% of individual rat granulosa cells which
responded to PGF2-alpha and GnRH responded to ANG II. Desensitization
(pretreatment) of rat granulosa cells by continuous hormone perifusio
n suggested that effects of PGF2-alpha, GnRH, and ANG II on [Ca2+]i we
re receptor specific. However, the effects of combined hormone treatme
nts on [Ca2+]i were not additive. The transient increase in [Ca2+]i in
response to PGF2-alpha or GnRH, alone, may be maximal. Results of thi
s study suggested that effects of PGF2-alpha, GnRH, and ANG II recepto
r-ligand interactions may be at least partially mediated by transient
increases in [Ca2+]i in rat granulosa cells. Similarly, effects of PGF
2-alpha, but not GnRH or ANG II, receptor-ligand interactions may be m
ediated by transient increases in [Ca2+]i in human granulosa cells.