MORPHOLOGICAL INVESTIGATION OF THE PORCINE LIVER DIRECTLY FOLLOWING PRESERVATION WITH EURO-COLLINS, UNIVERSITY-OF-WISCONSIN AND BRETSCHNEIDERS HTK SOLUTION
A. Aminalai et al., MORPHOLOGICAL INVESTIGATION OF THE PORCINE LIVER DIRECTLY FOLLOWING PRESERVATION WITH EURO-COLLINS, UNIVERSITY-OF-WISCONSIN AND BRETSCHNEIDERS HTK SOLUTION, Langenbecks Archiv fur Chirurgie, 377(2), 1992, pp. 81-88
In order to study perfusion effects of different liver preservation me
thods on liver structure, porcine livers were perfused with either Bre
tschneider's HTK (Histidine Tryptophane Ketoglutarat) solution. Euro-C
ollins (EC) solution or University of Wisconsin solution (UW) accordin
g to the respective recommended protocols. Subsequently, together with
a group of unprotected livers, all organs were examined by light and
electron microscopy including computer assisted morphometry. The width
of the space of Disse, the continuity of endothelial cells and the ul
trastructure of the hepatocytes were not impaired after cold perfusion
with any of the 3 solutions. However, we found considerable differenc
es between the groups with respect to removal of blood cells from live
r sinusoids. Livers flushed according to the HTK-protocol had the lowe
st residual blood cell content followed by the livers of the EC- and t
he UW-group. Centrilobular regions of the liver lobules were generally
better washed free of blood than periportal zones. Computer assisted
morphometry did not reveal any significant difference between the size
of hepatocytes of EC-, UW- and HTK livers. Only the hepatocytes of no
rmothermic control livers (biopsy samples) were 10% larger than hepato
cytes of cold flushed groups. None of the protective perfusion protoco
ls showed structural signs of perfusion injury.