EFFECTS OF TRANSFORMING GROWTH FACTOR-BETA-1 ON HUMAN PULMONARY ADENOCARCINOMA CELL-ADHESION, MOTILITY, AND INVASION INVITRO

Background: Transforming growth factor-beta-1 (TGF-beta-1), a potent g rowth modulator produced by a variety of tumor cells, as well as by pl atelets, has pleiotropic effects on cell-extracellular matrix interact ions and may influence tumor cell invasion and metastasis. Purpose: Ou r purpose was to characterize the effects of TGF-beta-1 on the adhesio n, motility, and invasiveness of a metastatic human pulmonary carcinom a (A549 cell line) in vitro. Methods: A549 cells were seeded onto type I collagen gels, and invasion over a 9-day period was measured in the presence or absence of TGF-beta-1 (0.1-10 ng/mL). In addition, cell a dhesion to substrata coated with type I collagen (1-100 nM) as well as haptotactic migration through filters coated with type I collagen (10 0-mu-g/mL) were measured following a 24-hour treatment with TGF-beta-1 (1-10 ng/mL). Results: TGF-beta-1 stimulated the invasion of A549 cel ls into type I collagen gels in a dose-dependent manner. Both the numb er of cells entering the gel and the depth of invasion into the gel we re increased. In addition, the effects of TGF-beta-1 were blocked in a dose-dependent manner by a purified polyclonal IgG against TGF-beta-1 but not by normal rabbit IgG. A549 cell invasion was accompanied by d ramatic changes in A549 cell morphology that included the appearance o f numerous long pseudopodia, consistent with a change in the motile be havior of these cells. TGF-beta-1 stimulated by approximately fourfold the haptotactic migration of A549 cells on polycarbonate filters coat ed with type I collagen. The TGF-beta-1-mediated increase in invasion and motility was accompanied by a fourfold increase in A549 cell adhes ion to type I collagen. Conclusions: The results suggest that TGF-beta -1 can influence cellular recognition of extracellular matrix componen ts and can modulate cellular adhesion and migration on these component s, leading to increased invasive potential. Implications: Given the wi despread tissue distribution of TGF-beta-1 and its secretion by a vari ety of tumor cells as well as by platelets, TGF-beta-1 may be an impor tant autocrine-paracrine regulator of the invasive phenotype in vivo.