SITE-SPECIFIC INTEGRATION OF THE HAEMOPHILUS-INFLUENZAE BACTERIOPHAGEHP1 - IDENTIFICATION OF THE POINTS OF RECOMBINATIONAL STRAND EXCHANGEAND THE LIMITS OF THE HOST ATTACHMENT SITE
Ma. Hauser et Jj. Scocca, SITE-SPECIFIC INTEGRATION OF THE HAEMOPHILUS-INFLUENZAE BACTERIOPHAGEHP1 - IDENTIFICATION OF THE POINTS OF RECOMBINATIONAL STRAND EXCHANGEAND THE LIMITS OF THE HOST ATTACHMENT SITE, The Journal of biological chemistry, 267(10), 1992, pp. 6859-6864
Isotopic transfer experiments and boundary replacement studies were us
ed to define the size and cleavage points of the Haemophilus influenza
e attB site for phage HP1 integration. The points of strand cleavage a
nd transfer were separated by 5' extensions with a spacing or overlap
region most probably 7 residues long. The complete HP1 attB site is in
cluded within an 18-base pair (bp) sequence surrounding the cleavage s
ites. The sequence of HP1 attB is remarkably symmetric. Two 8-bp inver
ted repeats surround the central residue of the 7-bp overlap sequence;
this central residue is the second residue of the anticodon sequence
of the H. influenzae tRNA(leu)(UUR) gene which contains attB, and this
symmetric segment encodes the anticodon stem and loop.