OVER-EXPRESSION OF PROTEIN-KINASE C-ALPHA ENHANCES PLATELET-DERIVED GROWTH FACTOR-INDUCED AND PHORBOL ESTER-INDUCED BUT NOT CALCIUM IONOPHORE-INDUCED FORMATION OF PROSTAGLANDINS IN NIH 3T3 FIBROBLASTS
G. Finkenzeller et al., OVER-EXPRESSION OF PROTEIN-KINASE C-ALPHA ENHANCES PLATELET-DERIVED GROWTH FACTOR-INDUCED AND PHORBOL ESTER-INDUCED BUT NOT CALCIUM IONOPHORE-INDUCED FORMATION OF PROSTAGLANDINS IN NIH 3T3 FIBROBLASTS, FEBS letters, 321(1), 1993, pp. 11-14
Over-expression of human protein kinase C-alpha in murine NIH 3T3 fibr
oblasts is associated with an increased platelet-derived growth factor
- and phorbol ester-mediated formation of prostaglandins, whereas the
calcium ionophore-induced release of arachidonic acid metabolites is u
naffected; however, the differences of arachidonic acid and prostaglan
din formation are much more pronounced with platelet-derived growth fa
ctor than with phorbol ester. Platelet-derived growth factor induces a
n identical elevation of intracellular free calcium in control and pro
tein kinase C-alpha over-expressing cells; the phorbol ester has no ef
fect on intracellular free calcium in both cell lines. These results d
emonstrate that protein kinase C-alpha may couple to arachidonic acid
cascade in NIH 3T3 fibroblasts.