A NEW PROCEDURE FOR THE ISOLATION OF ANTI-HIV COMPOUNDS (POLYSACCHARIDES AND POLYPHENOLS) FROM THE MARINE ALGA FUCUS-VESICULOSUS

Anti-HIV-active polysaccharides and polyphenols were isolated from the brown seaweed Fucus vesiculosus by hot H2O extraction of both the int act and the homogenized algae. This was followed by XAD2 chromatograph y and by sequential precipitation of the nonadsorbed compounds with gl acial HOAc and thereafter with EtOH. The precipitate was solubilized, dialyzed against distilled H2O, and chromatographed on SP-Sephadex C25 and on QAE-Sephadex A25. This was followed by gel filtration on Sepha dex G50 and Sephadex G100 and finally by hplc on a Shodex Ionpak S-804 column. For comparison, the commercial product fucoidan, a sulfated a lgal polysaccharide, was also further purified by the chromatographic techniques mentioned above. The isolated freeze-dried fractions obtain ed by these procedures were tested for inhibition of both HIV-induced syncytium formation and HIV reverse transcriptase enzyme activity. Som e of these fractions inhibited both of these activities at concentrati ons that were not cytotoxic.