Lr. Krumholz et al., PROPOSAL OF QUINELLA-OVALIS GEN-NOV, SP-NOV, BASED ON PHYLOGENETIC ANALYSIS, International journal of systematic bacteriology, 43(2), 1993, pp. 293-296
Quin's oval is a relatively large bacterium often seen in the rumens o
f sheep fed diets containing some readily fermented carbohydrates. It
has not been obtained in axenic cultures, but a number of its features
have been determined by various methods, such as studying cell suspen
sions purified from rumen fluid by differential centrifugation. We obt
ained similarly purified suspensions from a sheep fed a diet containin
g a large amount of molasses. Nearly complete 16S rRNA sequence analys
is of these cells as well as cells of Selenomonas ruminantium subsp. r
uminantium GA192 (ATCC 12561; type strain) and S. ruminantium subsp. l
actilytica HD4 (ATCC 27209) was done. These sequences were compared wi
th those of other bacteria. Evolutionary distance estimates indicated
that Quin's oval was most closely related to the Selenomonas-Megasphae
ra-Sporomusa group in the gram-positive phylum but that it belongs in
a new genus. We propose the name Quinella ovalis gen. nov., sp. nov.,
with its description based on previously known features.