IDENTIFICATION OF 2-AMINO-2,6-DIDEOXY-D-GLUCOSE (D-QUINOVOSAMINE), ISOLATED FROM THE CELL-WALLS OF THE ALKALIPHILIC BACILLUS-SP Y-25, BY 500-MHZ H-1-NMR SPECTROSCOPY

Cell walls of alkaliphilic Bacillus strain Y-25 are composed of gamma- peptidoglycan and two acidic polymers. An amino sugar, which was a mai n component of one acidic polymer, did not correspond to any of the co mmercially available hexosamines. The amino sugar was isolated from th e hydrolysate of the acidic polymer, purified, and identified as D-qui novosamine (2-amino-2,6-dideoxy-D-glucose) by 500-MHz NMR spectroscopi c analysis and polarimetry.