G. Orhan et al., THE EFFECT OF THE MALE CONTRACEPTIVE AGENT GOSSYPOL ACETIC-ACID ON MOUSE BONE-MARROW CELLS INVIVO - MICRONUCLEI AND MITOTIC INDEX, Contraception, 47(4), 1993, pp. 377-385
In this work the genotoxic effect of gossypol acetic acid (gossypol) w
as evaluated by determining the frequency of micronuclei and mitotic i
ndex in male mouse bone marrow cells in vivo. Bone marrow cells were c
ollected at 24th hour after the single intraperitoneal (20, 40, and 80
mug/g) administration of gossypol. Polychromatic erythrocytes (PCEs)
in the bone marrow were then evaluated with respect to micronuclei fre
quency. The dose-dependent increase in the micronuclei frequency was o
bserved. However, when compared with the control group, the increase w
as not found to be significiant (P>0.05). Also the mitotic index value
s were not found to be different from those control values (P>0.05). T
he results suggest that gossypol is not a clastogenic and mutagenic ag
ent in mouse bone marrow cells in vivo.