A physiologic fluctuation in threshold levels exists in automated peri
metry, which is greater in glaucoma and ocular hypertensive patients t
han in normal subjects. Fluctuation increases with greater eccentricit
y from fixation and in areas of reduced retinal sensitivity. Other fac
tors related to automated perimetry testing potentially may influence
threshold fluctuation including: a learning effect, reliability, pupil
size, age, and the mode of stimulus presentation. Statistical softwar
e may aid in analyzing both the single field and the changes between s
uccessive fields. However, statistical programs cannot replace physici
an judgment, and all factors that may influence threshold levels shoul
d be considered when interpreting automated visual fields.