TAKE OF RHESUS-HUMAN REASSORTANT TETRAVALENT ROTAVIRUS VACCINE IN BREAST-FED INFANTS

Rhesus-human reassortant tetravalent rotavirus vaccine at a titer of 4 x 10(4) plaque forming units was evaluated for immmunogenicity in 194 6-8-week-old breast-fed Turkish infants. The vaccine was administered orally as a single dose following either a meal of breast milk or 30 ml of sodium bicarbonate-buffered soy milk formula. Four-fold or great er responses in rotavirus IgA ELISA antibody were detected in 62% and 65% of the infants in the two groups, respectively (p=0.62). In a smal ler comparison group of non-breast-fed infants, an IgA response was de tected in 7 of 11 (64%) cases. In all vaccinees, a serological respons e was detected in 72% of the initially seronegative and 47% of the ini tially seropositive infants (p=0.001). We conclude that the take of rh esus-human reassortant tetravalent rotavirus vaccine in breast-fed inf ants is not compromised by breast feeding before vaccination. However, a higher titered preparation of the same vaccine may be required to i mprove overall immunogenicity in young infants, particularly in those with pre-vaccination rotavirus antibody.