ONLINE CHEMILUMINESCENCE ASSAY USING FIA AND FIBER OPTICS FOR URINARYAND BLOOD-GLUCOSE

A chemiluminescence fiber optic system coupled to flow injection analy sis (FIA) and ion exchange chromatography has been developed for deter mining glucose in blood and urine. Immobilized glucose oxidase acted o n beta-D-glucose to produce hydrogen peroxide, which was then reacted with luminol in the presence of ferricyanide to produce a light signal . Endogenous ascorbic acid and uric acid present in urine or blood sam ples were effectively retained by an upstream acetate anion exchanger. In addition, acetaminophen could also be adsorbed by this ion exchang er. The detection system exhibited a sensitivity of 1.315 +/- 0.044 RU muM-1 for glucose with a minimum detection level of 1 muM. When appli ed for the determination of urinary and blood glucose levels, the resu lts obtained compared well with those of the reference hexokinase assa y. Immobilized glucose oxidase was reused for over 500 analyses withou t losing its original activity. A conservative estimate for the reuse of the acetate ion exchange column was about 100 analyses.