P53 GENE MUTATION IN PRIMARY HUMAN RENAL-CELL CARCINOMA

We searched for possible mutations in the entire coding region of tumo r suppressor gene p53 in primary human renal cell carcinomas using pol ymerase chain reaction and single-strand conformational polymorphism a nalysis of RNA. We found p53 mutations in 2 of 21 cases (10%). DNA seq uencing of the polymerase chain reaction products verified that the fi rst case included a 17-base deletion at the beginning of exon 6. The s econd case showed a T to C transition at nucleotide 1328 in exon 7. No clinical or pathological similarity was found in the renal cell carci nomas containing the mutated p53 genes. Present results suggest that p 53 mutation is involved at low frequency in human renal cell carcinoma s.