XENIC CULTURE OF STEINERNEMA-KUSHIDAI (NEMATODA, STEINERNEMATIDAE) ONARTIFICIAL MEDIA

S. kushidai was xenically propagated on dog food/agar and pig offal/ag ar media supplemented with peptone (1.2%). It propagated to a lesser e xtent on Wouts' lipid agar medium. The nematode was also xenically pro pagated on a medium of soluble starch, D-glucose, lard, peptone, yeast extract, agar and distilled water. pH and media composition required for optimal production of the nematode were examined. Maximum yield of infective juveniles was on soluble starch (0.6%), D-glucose (1.0%), l ard (3.0%), peptone (1.5%), yeast extract (1.5%), agar (0.3 to 1.0%) a nd 33 mM Bis-Tris buffer (pH 6.7).