ORGANOCHROMIUM COMPLEXES LABELED AMINOGLYCOSIDE ANTIBIOTICS DERIVED FROM KANAMYCIN-A AND TOBRAMYCIN - SYNTHESIS, STRUCTURAL CHARACTERIZATION AND USE AS METALLOTRACERS FOR IMMUNOASSAYS

The synthesis of metallocenic derivatives of aminoglycoside antibiotic s, i. e. kanamycin A and tobramycin, is described. The organometallic- labelled compounds have been obtained from the reaction between the po lyamino-drugs and organochromium esters of N-hydroxysuccinimide. The r eaction proceeded selectively at the 6'-N position, as might be deduce d both from the mass and the pH-dependent C-13-NMR spectra. The proced ure could be regarded as generally useful for the metallolabelling of aminoglycoside antibiotics. As an example of application a competitive immunoassay based on the use of these labels is proposed.