A GENERAL-METHOD OF CURVE FITTING AND ERROR ANALYSIS USING A SPREADSHEET - DETERMINATION OF THE BINDING CONSTANTS OF TIGHT-BINDING LIGANDS IN VARIABLE VOLUME ASSAYS

Authors
DELAHUNTY MD MACK JPG
Citation
Md. Delahunty et Jpg. Mack, A GENERAL-METHOD OF CURVE FITTING AND ERROR ANALYSIS USING A SPREADSHEET - DETERMINATION OF THE BINDING CONSTANTS OF TIGHT-BINDING LIGANDS IN VARIABLE VOLUME ASSAYS, Computer applications in the biosciences, 9(2), 1993, pp. 127-131
Citations number
12
Journal title
Computer applications in the biosciences → ACNP
ISSN journal
02667061
Volume
9
Issue
2
Year of publication
1993
Pages
127 - 131
Database
ISI
SICI code
0266-7061(1993)9:2<127:AGOCFA>2.0.ZU;2-H
Abstract
A generalized iterative method of curve fitting and error estimation i s described, using a spreadsheet with Monte Carlo simulations. The met hod is demonstrated on an equilibrium binding experiment with reverse transcriptase of human immunodeficiency virus-1, in which the concentr ation of all reactants, including enzyme, can vary arbitrarily and any number of ligands can bind tightly.