A HEAT-LABILE SERINE PROTEINASE FROM PENICILLIUM-CITRINUM

Authors
YAMAMOTO N MATSUMOTO K YAMAGATA Y HIRANO KI ICHISHIMA E
Citation
N. Yamamoto et al., A HEAT-LABILE SERINE PROTEINASE FROM PENICILLIUM-CITRINUM, Phytochemistry, 32(6), 1993, pp. 1393-1397
Citations number
33
Journal title
Phytochemistry → ACNP
ISSN journal
00319422
Volume
32
Issue
6
Year of publication
1993
Pages
1393 - 1397
Database
ISI
SICI code
0031-9422(1993)32:6<1393:AHSPFP>2.0.ZU;2-Z
Abstract
A serine proteinase from Penicillium citrinum was purified. The M(r) a nd isoelectric point were determined as about 26 000 and 9.5, respecti vely. Activity was retained up to above 40-degrees at pH 7 for 30 min, but the enzyme was completely inactivated at 50-degrees. The first am ino acids in the N-terminal region were ANVVQSNVPSWGLARISSK-RPGTTSYTYD STAGEGVVFYGVDTG. The specificity differs from that of other serine pro teinases. Kinetic studies on fluorogenic substrates were determined.