ELECTRO-IMMORTALIZATION OF B-LYMPHOCYTES ISOLATED FROM STOMACH CARCINOMA BIOPSY MATERIAL

The advantages of electrofusion were used to immortalise the small num ber of B-cells from fresh biopsy material taken from a gastric carcino ma of a patient. Two stable human antibody secreting clones could be p roduced which exhibited functional activity against the autologous tum our cells (inhibition of cell adhesion and immunofluorescence staining of the membranes). This ShoWS that a variety of hitherto inacessible B lymphocyte populations from other human organ biopsies can be immort alised by the improved electrofusion technique.