Zh. Huang et al., CHARACTERIZATION OF N-ETHOXYCARBONYL ETHYL-ESTERS OF AMINO-ACIDS BY MASS-SPECTROMETRY, Journal of chromatography, 635(2), 1993, pp. 271-281
Derivatization of amino acids by using ethyl chloroformate-ethanol-pyr
idine provides volatile products, N-ethoxycarbonyl amino acid ethyl es
ters (ECEEs), which are easily amenable to GC or GC-MS analysis. MS be
havior of these compounds under electron-impact has been studied. The
fragments observed in the spectra facilitate recognition of commonly o
ccurring protein amino acids and characterization of unknown analogues
.