CAPILLARY ELECTROPHORETIC CHIRAL SEPARATIONS WITH CYCLODEXTRIN ADDITIVES .1. ACIDS - CHIRAL SELECTIVITY AS A FUNCTION OF PH AND THE CONCENTRATION OF BETA-CYCLODEXTRIN FOR FENOPROFEN AND IBUPROFEN
Yy. Rawjee et al., CAPILLARY ELECTROPHORETIC CHIRAL SEPARATIONS WITH CYCLODEXTRIN ADDITIVES .1. ACIDS - CHIRAL SELECTIVITY AS A FUNCTION OF PH AND THE CONCENTRATION OF BETA-CYCLODEXTRIN FOR FENOPROFEN AND IBUPROFEN, Journal of chromatography, 635(2), 1993, pp. 291-306
An equilibrium model has been developed to describe the pH and cyclode
xtrin concentration dependence of the electrophoretic mobilities as we
ll as the chiral selectivities observed during the capillary electroph
oretic separation of the enantiomers of weak acids. The parameters of
the model can be readily derived from three specific sets of capillary
electrophoretic experiments: cyclodextrin-free background electrolyte
s of varying pH values are used in the first set of experiments, backg
round electrolytes with the same high pH but varying concentrations of
cyclodextrin are used in the second set, and background electrolytes
of the same low pH but with varying concentrations of cyclodextrin are
used in the third set of experiments. The model has been tested with
fenoprofen and ibuprofen as model substances and beta-cyclodextrin as
resolving agent, and an excellent agreement has been found between the
calculated and the measured values. Baseline separations have been ac
hieved for the enantiomers of both fenoprofen and ibuprofen in less th
an thirty minutes.