De. Hughes et P. Richberg, CAPILLARY MICELLAR ELECTROKINETIC, SEQUENTIAL MULTIWAVELENGTH CHROMATOGRAPHIC CHARACTERIZATION OF A CHIMERIC MONOCLONAL-ANTIBODY CYTOTOXIN CONJUGATE, Journal of chromatography, 635(2), 1993, pp. 313-318
An analysis of a doxorubicin-conjugated chimeric antibody has been dev
eloped by micellar electrokinetic capillary chromatography. The proced
ure separates the conjugated antibody, the antibody light and heavy ch
ains, antibody fragments, and unconjugated doxorubicin. The protein-de
rived species are monitored at 280 nm and the doxorubicin bearing spec
ies are monitored at 500 nm. Consistency with the proposed in vivo mec
hanism of action of the antibody conjugate in acid media is demonstrat
ed.