CAPILLARY MICELLAR ELECTROKINETIC, SEQUENTIAL MULTIWAVELENGTH CHROMATOGRAPHIC CHARACTERIZATION OF A CHIMERIC MONOCLONAL-ANTIBODY CYTOTOXIN CONJUGATE

An analysis of a doxorubicin-conjugated chimeric antibody has been dev eloped by micellar electrokinetic capillary chromatography. The proced ure separates the conjugated antibody, the antibody light and heavy ch ains, antibody fragments, and unconjugated doxorubicin. The protein-de rived species are monitored at 280 nm and the doxorubicin bearing spec ies are monitored at 500 nm. Consistency with the proposed in vivo mec hanism of action of the antibody conjugate in acid media is demonstrat ed.