OXIDANT STRESS AND GLOMERULAR PROSTANOID PRODUCTION - INFLUENCE OF ANGIOTENSIN-CONVERTING ENZYME-INHIBITION

1. The effect of H2O2 (4.7 x 10(-9)-4.7 x 10(-3) M) on prostanoid prod uction by isolated glomeruli from normotensive (WKY) and, spontaneousl y hypertensive rats (SHR) has been studied. 2. Oxidant stress signific antly increased synthesis of prostaglandin E2 (PGE2), I2 (PGI2) and th romboxane A2 (TxA2) by glomeruli from both strains whereas the ratio ( PGE2 + PGI2)/TxA2 increased in only SHR. 3. Pre-incubation of glomerul i with the angiotensin converting enzyme inhibitors captopril or lisin opril, had virtually no effect on H2O2-induced synthesis of individual prostanoids nor on the ratio (PGE2 + PGI2)/TxA2 by glomeruli from eit her WKY or SHR. 4. The findings suggest that H2O2-induced changes in g lomerular function may be mediated, in part, by PGs but fail to suppor t the suggestion that the ability of ACEI to protect glomeruli from H2 O2-induced damage is determined by PGs.