THE ALPHA-ANEURYSM - A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE

Authors
KEEFE LJ SONDEK J SHORTLE D LATTMAN EE
Citation
Lj. Keefe et al., THE ALPHA-ANEURYSM - A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE, Proceedings of the National Academy of Sciences of the United Statesof America, 90(8), 1993, pp. 3275-3279
Citations number
26
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
90
Issue
8
Year of publication
1993
Pages
3275 - 3279
Database
ISI
SICI code
0027-8424(1993)90:8<3275:TA-ASM>2.0.ZU;2-I
Abstract
The x-ray crystal structure of a mutant of staphylococcal nuclease tha t contains a single glycine residue inserted in the C-terminal alpha-h elix has been solved to 1.67 angstrom resolution and refined to a crys tallographic R value of 0.170. This inserted glycine residue is accomm odated in the alpha-helix by formation of a previously uncharacterized bulge, which we term the alpha aneurism. A conformational search of k nown protein structures has identified the alpha aneurism in a number of protein families, including the histocompatibility antigens and hem oglobins.