EXPRESSION OF SIMIAN VIRUS-40 LARGE-T (TUMOR) ONCOGENE IN MOUSE CHONDROCYTES INDUCES CELL-PROLIFERATION WITHOUT LOSS OF THE DIFFERENTIATED PHENOTYPE

Authors
MALLEINGERIN F OLSEN BR
Citation
F. Malleingerin et Br. Olsen, EXPRESSION OF SIMIAN VIRUS-40 LARGE-T (TUMOR) ONCOGENE IN MOUSE CHONDROCYTES INDUCES CELL-PROLIFERATION WITHOUT LOSS OF THE DIFFERENTIATED PHENOTYPE, Proceedings of the National Academy of Sciences of the United Statesof America, 90(8), 1993, pp. 3289-3293
Citations number
56
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
90
Issue
8
Year of publication
1993
Pages
3289 - 3293
Database
ISI
SICI code
0027-8424(1993)90:8<3289:EOSVL(>2.0.ZU;2-5
Abstract
We have infected primary embryonic mouse limb chondrocytes with a retr ovirus carrying simian virus 40 early regions and have obtained a mono clonal mouse chondrocyte line, MC615, that was able to grow on culture dishes for at least 7 months and 20 passages. MC615 cells show expres sion of simian virus 40 large T (tumor) antigen and express markers ch aracteristic of cartilage in vivo, such as types II, IX, and XI collag en, as well as cartilage aggrecan and link protein. These data show th at cell growth induced by large T oncogene expression does not prevent the maintenance of the chondrocytic phenotype.