GLYOXALASE ACTIVITIES IN HUMAN TUMOR-CELL LINES INVITRO

The activities of glyoxalase I (EC 4.4.1.5) and glyoxalase II (EC 3.1. 2.6) were measured in 3 human non-malignant and 28 tumour cell lines. The activity of glyoxalase I was in the range 321-8751 mUnits/mg of pr otein. The glyoxalase I/glyoxalase II activity ratio was in the range 7-147, glyoxalase I activity was always markedly greater than glyoxala se activity. There was no significant difference between glyoxalase ac tivities in non-malignant and tumour cell lines. The activity of glyox alase I was within and above the range found in normal human tissues a nd the activity of glyoxalase II was within the range and below that f ound in normal human tissues.