MAPPING OF DERIVATIZED BIOGENIC-AMINES BY 2-DIMENSIONAL THIN-LAYER CHROMATOGRAPHY - A COMPARATIVE-STUDY

The analysis of amines is greatly enhanced by the formation of derivat ives with improved chromatographic properties. We have used dansyl chl oride, dabsyl chloride, and 7-chloro-4-nitrobenzoxazole to derivatise fourteen structurally diverse amines which occur in biological systems , and have made a comparative study of their thin-layer chromatographi c properties. Two-dimensional TLC maps of derivatised amines are prese nted for the three different procedures, and some factors which influe nce sensitivity are evaluated. On grounds of sensitivity of detection and chemical stability of the derivatives formed, dansyl chloride is t he preferred reagent for chromatographic analysis of complex mixtures of biogenic amines.