EFFECT OF ACCESSORY CELLS ON STIMULATION OF MURINE T-CELL LEUKEMIA WITH ANTIBODIES TO THE CD3 T-CELL ANTIGEN RECEPTOR COMPLEX

Stimulation of EL4 and RL male 1 leukemia cells in vitro with immobili zed anti-CD3epsilon monoclonal antibody (mAb) (145-2C11) or anti-TCRbe ta mAb (H57-597) in the absence of accessory cells induced interleukin -2 (IL-2) production, and caused growth inhibition. The growth inhibit ion was, however, transient and the tumors started to grow again withi n 5 days in immobilizing plates treated with antibodies at concentrati ons of 2.5-100 mug/ml. Addition of mitomycin C-treated accessory cells to the culture inhibited IL-2 production and resulted in augmented an d persistent growth inhibition. No recovery of tumor growth was observ ed. Furthermore, DNA from EL4 and RL male 1 leukemia cells stimulated with anti-CD3/TCR mAbs was fragmented even in the absence of accessory cells, but fragmentation was much greater in the presence of accessor y cells. Marginal and high expression of the bcl-2 gene were observed in EL4 and RL male 1, respectively, indicating that apoptosis of these leukemias mediated by signalling through the CD3/TCR complex has no d irect relationship with expression of the bcl-2 gene.