The net photosynthetic rate (P(N)), intercellular CO2 concentration (C
(i)), transpiration rate (E), stomatal resistance (r(s)), and water po
tential (PSI(W)) of a C3 grass (Kentucky bluegrass, Poa pratensis L.)
and a C4 grass (big bluestem, Andropogon gerardii Vitman) growing in t
he spring in a tallgrass prairie under two levels of CO2 (ambient and
twice ambient) were compared. Elevated CO2 (HC) increased P(N) of Kent
ucky bluegrass (C3) by 47.0 % but did not affect P(N) of big bluestem
(C4). HC increased C(i) of both grasses by about the same amount (is-a
pproximately-equal-to cm3 m-3), but reduced E (and parallelly increase
d r(s)) of big bluestem more than those of Kentucky bluegrass. HC incr
eased PSI(W) of both grasses by about 30 %. Kentucky bluegrass had a l
ower PSI(W) than big bluestem, but HC increased PSI(W) of Kentucky blu
egrass to values more similar to those of big bluestem under ambient C
O2 (LC). Hence a high PSI(W), resulting from HC, was necessary for a h
igh P(N).