THE HISTOCHEMICAL PROFILES OF FAST FIBER TYPE-IIB, TYPE-IID, AND TYPE-IIA IN SKELETAL-MUSCLES OF MOUSE, RAT, AND RABBIT

Citation
N. Hamalainen et D. Pette, THE HISTOCHEMICAL PROFILES OF FAST FIBER TYPE-IIB, TYPE-IID, AND TYPE-IIA IN SKELETAL-MUSCLES OF MOUSE, RAT, AND RABBIT, The Journal of histochemistry and cytochemistry, 41(5), 1993, pp. 733-743
Citations number
28
ISSN journal
00221554
Volume
41
Issue
5
Year of publication
1993
Pages
733 - 743
Database
ISI
SICI code
0022-1554(1993)41:5<733:THPOFF>2.0.ZU;2-6
Abstract
This study characterized histochemically three fast fiber types (IIB, IID, IIA) in skeletal muscles of mouse, rat, and rabbit, with special reference to fiber types IIB and IID. The results are complemented by biochemical analyses of myosin heavy chain composition in these muscle s. Fiber type delineation is based on various methods for mATPase stai ning with preincubations and assays under different conditions. In rat and mouse, IIB and IID fibers can be best distinguished according to their different mATPase stabilities towards formaldehyde and alkaline pH. In rabbit, the method of Matoba and Gollnick using acid pre-incuba tion provided best and most reproducible results. In addition to their different mATPase stabilities, the three fast fiber types differ with regard to their oxidative capacities and cross-sectional fiber areas in the three species. In general, Type IIB fibers are the largest and least oxidative, Type IIA fibers the smallest and most oxidative, and Type IID fibers intermediate. In rabbit, Type IID fibers are the predo minant fast fiber population in extensor digitorum longus, psoas, and tibialis anterior muscles. As judged from histochemistry, these muscle s of rabbit do not contain pure Type IIB fibers. This is in accordance with biochemical results that show the HCIId to form the majority of the myosin heavy chain complement expressed in these muscles. On the o ther hand, IIB fibers are numerous in rabbit adductor magnus, gastrocn emius, and vastus lateralis muscles. Similarly, appreciable amounts of myosin heavy chain HCIIb are found in the three latter muscles of rab bit.