MODULATION OF INVIVO IMMUNE-RESPONSE BY SELECTIVE DEPLETION OF NEUTROPHILS USING A MONOCLONAL-ANTIBODY, RP-3 .2. INHIBITION BY RP-3 TREATMENT OF MONONUCLEAR LEUKOCYTE RECRUITMENT IN DELAYED-TYPE HYPERSENSITIVITY TO SHEEP RED-BLOOD-CELLS IN RATS

We demonstrated in our previous paper that treatment of rats with RP-3 , a mAb that depletes in vivo neutrophils selectively, resulted in inh ibition of both the priming and effector phases of delayed type hypers ensitivity (DTH) to SRBC. In order to clarify the mechanisms of this p henomenon, we examined the effect of RP-3 treatment on mononuclear leu kocyte (MNL) recruitment in DTH. When rats had been treated with RP-3 at the time of Ag priming, MNL migration, which accompanies DTH, was i nhibited. The prior infiltration of neutrophils was also partially req uired for MNL recruitment because migration was inhibited when the imm une rats were treated with RP-3 at the time of DTH elicitation.