Research examining the relationship between beta-endorphin immunoreact
ivity and affect has resulted in equivocal findings. To examine this r
elationship further, 10 male and 10 female college-age students partic
ipated in a controlled free-weight exercise session. Blood plasma samp
les were procured both prior to and following the exercise bout and be
ta-endorphin levels were determined by immunoassay. Analysis indicated
that plasma beta-endorphin levels following exercise were significant
ly decreased from pre-exercise levels. Pearson correlations showed no
significant relationship between pre- or postexercise plasma beta-endo
rphins and either total mood disturbance or Profile of Mood States sub
scores. The data support previous failure of resistance exercise to pr
oduce an increase in beta-endorphin immunoreactivity as well as the la
ck of a significant relationship between affect and the beta-endorphin
response to exercise.