EFFECT OF CYCLOSPORINE-A AND ITS METABOLITES AND ANALOGS ON LIPID-PEROXIDATION IN RABBIT RENAL MICROSOMES

Rabbit renal microsomes were used to investigate the effect on lipid p eroxidation (LPO) of cyclosporin A (CsA) and its first generation meta bolites Ml, M17 and M21, and of two natural CsA analogs: cyclosporins C and D (CsC & CsD respectively), at concentrations of 1 to 10 mug/ml. No induction of lipid peroxidation was observed with these substances except CsA at a high level (10 mug/ml); but at a concentration of CsA that would be reached in vivo, no inductive effect on LPO was noted. A moderate inhibitory effect upon LPO was observed with CsC and M21, b ut its biological significance is questionable.