S. Yamamoto et al., AVIDITY OF ANTIBODY AND AGGLUTINABILITY OF ANTIBODY-SENSITIZED LATEX IN LATEX AGGLUTINATION-TEST, Veterinary immunology and immunopathology, 36(3), 1993, pp. 257-264
This paper describes the avidity of IgG antibody used for preparation
of latex sensitized with IgG antibody (IgG-sensitized latex) and the a
gglutinability of IgG-sensitized latex in slide reversed passive latex
agglutination (RPLA). Using immunodiffusion techniques, it was found
that anti-canine C-reactive protein (CRP) sera from four rabbits immun
ized with canine CRP had the same antibody titers. However, the antibo
dies had different levels of avidity. When lattices were sensitized un
der the same condition with the IgG antibodies of different avidity le
vels separated from the above-mentioned antisera using Protein A and c
anine CRP-Sepharose 4B immunosorbent, these demonstrated different pat
terns of agglutinability in slide RPLA. The latex sensitized with IgG
antibody of higher avidity demonstrated a stronger agglutinability.