AVIDITY OF ANTIBODY AND AGGLUTINABILITY OF ANTIBODY-SENSITIZED LATEX IN LATEX AGGLUTINATION-TEST

This paper describes the avidity of IgG antibody used for preparation of latex sensitized with IgG antibody (IgG-sensitized latex) and the a gglutinability of IgG-sensitized latex in slide reversed passive latex agglutination (RPLA). Using immunodiffusion techniques, it was found that anti-canine C-reactive protein (CRP) sera from four rabbits immun ized with canine CRP had the same antibody titers. However, the antibo dies had different levels of avidity. When lattices were sensitized un der the same condition with the IgG antibodies of different avidity le vels separated from the above-mentioned antisera using Protein A and c anine CRP-Sepharose 4B immunosorbent, these demonstrated different pat terns of agglutinability in slide RPLA. The latex sensitized with IgG antibody of higher avidity demonstrated a stronger agglutinability.