S. Yamamoto et al., EFFICIENT PREPARATION OF MONOSPECIFIC ANTI-CANINE C-REACTIVE PROTEIN SERUM AND PURIFICATION OF CANINE C-REACTIVE PROTEIN BY AFFINITY-CHROMATOGRAPHY, Veterinary immunology and immunopathology, 36(3), 1993, pp. 293-301
The canine C-reactive protein (CRP) fraction isolated from canine acut
e-phase serum on a phosphorylcholine-Sepharose 4B column was further s
ubjected to Sephacryl S-300 gel filtration. A canine CRP fraction not
containing IgM was then obtained. The antisera, obtained after several
immunizations with this canine CRP fraction, contained nonspecific an
tibodies that reacted with albumin, transferrin and IgG in addition to
CRP. This antiserum could be easily changed to monospecific canine CR
P serum, when it was subjected to absorption for only 15 min using glu
taraldehyde-insolubilized normal canine serum protein containing 3.5 m
ug ml-1 of CRP. Pure canine CRP was isolated with a recovery rate of 9
5% from canine acute-phase serum by affinity chromatography using spec
ific anti-canine CRP antibody.