ANALYSIS OF CYCLIC NUCLEOTIDE-RELATED ENZYMES BY CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY

Authors
LANGRIDGE JI BRENTON AG WALTON TJ HARRIS FM NEWTON RP
Citation
Ji. Langridge et al., ANALYSIS OF CYCLIC NUCLEOTIDE-RELATED ENZYMES BY CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY, Rapid communications in mass spectrometry, 7(4), 1993, pp. 293-303
Citations number
17
Categorie Soggetti
Spectroscopy,"Chemistry Analytical
Journal title
Rapid communications in mass spectrometry → ACNP
ISSN journal
09514198
Volume
7
Issue
4
Year of publication
1993
Pages
293 - 303
Database
ISI
SICI code
0951-4198(1993)7:4<293:AOCNEB>2.0.ZU;2-3
Abstract
Continuous-flow fast-atom bombardment mass spectrometry has been devel oped to directly monitor cyclic nucleotide (substrate) and its product levels from an on-going phosphodiesterase reaction. Analysis of cAMP and cCMP phosphodiesterase incubates have been performed where the tem poral evolution of the enzymic reaction is monitored and the effect of enzyme concentration upon the rate of reaction determined. Quantitati ve data on the enzyme kinetics have been obtained, in the form of Line weaver-Burke plots, that are shown to correlate well with well-establi shed radiometric methods.