SIMULTANEOUS PIXE AND PIGE ANALYSIS OF METALLOPROTEINS SEPARATED BY CELLULOSE-ACETATE ELECTROPHORESIS

Authors
SZOKEFALVINAGY Z DEMETER I HOLLOSNAGY K RAISANEN J
Citation
Z. Szokefalvinagy et al., SIMULTANEOUS PIXE AND PIGE ANALYSIS OF METALLOPROTEINS SEPARATED BY CELLULOSE-ACETATE ELECTROPHORESIS, Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 75(1-4), 1993, pp. 165-168
Citations number
7
Categorie Soggetti
Physics, Nuclear","Nuclear Sciences & Tecnology","Instument & Instrumentation
Journal title
Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms → ACNP
ISSN journal
0168583X
Volume
75
Issue
1-4
Year of publication
1993
Pages
165 - 168
Database
ISI
SICI code
0168-583X(1993)75:1-4<165:SPAPAO>2.0.ZU;2-6
Abstract
The Fe and N distributions of myoglobin bands prepared by cellulose ac etate electrophoresis were simultaneously measured by detecting the pr oton induced Fe K X-rays and the gamma-rays of 2313 keV energy coming from the N-14(p, p'gamma)N-14 nuclear reaction at about 4 MeV proton e nergy. In the case of unstained bands the Fe and N distributions were very similar in shape and the integrated gamma- and X-ray yields were found to be proportional to the amounts of myoglobin supplied for elec trophoresis. In the cases of stained samples no quantitative conclusio ns could be drawn as a consequence of the drastic denaturation of the proteins caused by the staining.