CHARACTERIZATION OF WHEAT AEGILOPS-VENTRICOSA INTROGRESSION AND ADDITION LINES WITH RESPECT TO THE M(V) GENOME

Stable wheat-Aegilops introgression lines with 42 chromosomes (H-93), derived by repeated selfing from a cross (Triticum turgidum x Aegilops ventricosa) x T. aestivum, have been characterized using the followin g DNA probes and isozyme markers: (1) single or low-copy DNA fragments from Ae. ventricosa; (2) known cDNA probes corresponding to alpha1-th ionin, monomeric alpha-amylase inhibitor, the CM3 subunit of tetrameri c alpha-amylase inhibitor, and sucrose synthase from wheat; (3) anonym ous cDNA probes from wheat that have been mapped by Sharp et al. (1989 ); (4) isozyme markers corresponding to aconitase, shikimate dehydroge nase, adenylate kinase, and endopeptidase. Meiotic metaphases of appro priate hybrids involving selected H-93 lines have been investigated by the Giemsa C-banding technique. The substitution of whole chromosomes [(5A)5M(v); (4D)4M(v); (5D) 5M(v); (7D) 7M(v)] and chromosomal segmen ts (1M(v); 3M(v); 5M(v); 7M(v)) from the M(v) genome of Aegilops ventr icosa has been demonstrated. The distribution of selected markers amon g putative wheat-Ae. ventricosa addition lines has also been investiga ted. The 7M(v) addition has been characterized for the first time, whi le the identity of the previously reported 5M(v) and 6M(v) additions h as been confirmed.