GENETICS OF GLIADINS CODED BY THE GROUP-1 CHROMOSOMES IN THE HIGH-QUALITY BREAD WHEAT CULTIVAR NEEPAWA

The inheritance and biochemical properties of gliadins controlled by t he group 1 chromosomes of the high-quality bread wheat cultivar Neepaw a were studied in the progeny of the cross Neepawa x Costantino by six different electrophoretic procedures. Chromosome 1B of Neepawa contai ns two gliadin loci, one (Gli-B1) coding for at least six omega- or ga mma-gliadins, the other (Gli-B3) controlling the synthesis of gliadin N6 only. The map distance between these loci was calculated as 22.1 cM . Amongst the chromosome 1A gliadins, three proteins are encoded at th e Gli-A1 locus whereas polypeptides N-14-N15-N16 are controlled by a r emote locus which recombines with Gli-A1. Six other gliadins are contr olled by a gene cluster at Gli-D1 on chromosome 1D. Canadian wheat cul tivars sharing the Gli-Bi allele of Neepawa were found to differ in th e presence or absence of gliadin N6. The electrophoretic mobilities of proteins N6 and N14-N15-N16 were unaffected by the addition of a redu cing agent during two-dimensional sodium dodecyl sulphate polyacrylami d-gel electrophoresis, suggesting the absence of intra-chain disulphid e bonds in their structure.