H. Gersdorf et al., FLUORESCENCE INSITU HYBRIDIZATION FOR DIRECT VISUALIZATION OF GRAM-NEGATIVE ANAEROBES IN SUBGINGIVAL PLAQUE SAMPLES, FEMS immunology and medical microbiology, 6(2-3), 1993, pp. 109-114
Fluorescent oligonucleotide probes complementary to variable regions o
f Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA
were used to identify these organisms in smears of formaldehyde-fixed
subgingival plaque samples from patients suffering from periodontitis
. Fluorescence in situ hybridization represents a useful method for as
sessing the microbial ecology of the periodontal flora.