IDENTIFICATION OF MYCOBACTERIAL ISOLATES BY THIN-LAYER AND CAPILLARY GAS-LIQUID-CHROMATOGRAPHY UNDER DIAGNOSTIC ROUTINE CONDITIONS

Authors
YASSIN AF BINDER C SCHAAL KP
Citation
Af. Yassin et al., IDENTIFICATION OF MYCOBACTERIAL ISOLATES BY THIN-LAYER AND CAPILLARY GAS-LIQUID-CHROMATOGRAPHY UNDER DIAGNOSTIC ROUTINE CONDITIONS, Zentralblatt fur Bakteriologie, 278(1), 1993, pp. 34-48
Citations number
35
Categorie Soggetti
Microbiology,Virology
Journal title
Zentralblatt fur Bakteriologie → ACNP
ISSN journal
09348840
Volume
278
Issue
1
Year of publication
1993
Pages
34 - 48
Database
ISI
SICI code
0934-8840(1993)278:1<34:IOMIBT>2.0.ZU;2-J
Abstract
The mycolic acid patterns of 75 strains of mycobacteria belonging to 4 6 different species were studied by thin-layer chromatography (TLC). A dditionally, the mycolic acid pyrolytic cleavage products were determi ned by capillary gas-liquid chromatography (C-GLC). Eleven different p atterns based on number, type and R(f) values of the detected lipid sp ots were identified. The mycolic acid methyl ester profiles of clinica l isolates were compared with those of reference strains. In this way, it was possible to relate these clinical isolates to mycobacterial gr oups defined by their mycolic acid profiles. The 11 patterns and the m ethods used are described in detail.