Virgin German cockroach adult females release an attractant that can b
e extracted with organic solvents and trapped from air blown over sexu
ally receptive females. Behavioral assays with an olfactometer showed
that the attractant was produced exclusively by adult females and it e
licited behavioral responses in adult males, confirming its function a
s a female sex pheromone. Using behavioral and electrophysiological as
says, we localized the site of pheromone production on the tenth abdom
inal tergite where an adult female-specific gland is found. Females wh
ose glands were ablated were significantly less attractive to males th
an sham-operated control females.