A NEW MULTILABELING TECHNIQUE FOR SIMULTANEOUS DEMONSTRATION OF DIFFERENT ISLET CELLS IN PERMANENT SLIDES

Authors
POUR PM KAZAKOFF K DULANEY K
Citation
Pm. Pour et al., A NEW MULTILABELING TECHNIQUE FOR SIMULTANEOUS DEMONSTRATION OF DIFFERENT ISLET CELLS IN PERMANENT SLIDES, International journal of pancreatology, 13(2), 1993, pp. 139-142
Citations number
10
Categorie Soggetti
Endocrynology & Metabolism",Physiology
Journal title
International journal of pancreatology → ACNP
ISSN journal
01694197
Volume
13
Issue
2
Year of publication
1993
Pages
139 - 142
Database
ISI
SICI code
0169-4197(1993)13:2<139:ANMTFS>2.0.ZU;2-T
Abstract
We describe a simple immunohistochemical method for simultaneous demon stration of insulin, glucagon, somatostatin, and pancreatic polypeptid e cells of the Langerhans islet in routinely fixed paraffin-embedded h uman and Syrian hamster pancreases. With the use of alcohol insoluble chromogens, the slides can be mounted in Permount and be kept permanen tly without losing the color quality and intensity. With this techniqu e, the spatial topographical distribution and morphology of the indivi dual pancreatic endocrine cells can be investigated in the healthy and diseased pancreas.