CONTINUOUS PRODUCTION OF THERMOSTABLE ALPHA-AMYLASE BY IMMOBILIZED BACILLUS CELLS IN A FLUIDIZED-BED REACTOR

Authors
RAMAKRISHNA SV JAMUNA R EMERY AN
Citation
Sv. Ramakrishna et al., CONTINUOUS PRODUCTION OF THERMOSTABLE ALPHA-AMYLASE BY IMMOBILIZED BACILLUS CELLS IN A FLUIDIZED-BED REACTOR, Applied biochemistry and biotechnology, 37(3), 1992, pp. 275-282
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
37
Issue
3
Year of publication
1992
Pages
275 - 282
Database
ISI
SICI code
0273-2289(1992)37:3<275:CPOTAB>2.0.ZU;2-4
Abstract
The exponential cells of Bacillus cereus immobilized in calcium algina te and spun into microcylindrical particles were used in a fluidized-b ed reactor for continuous synthesis of thermostable alpha-amylase. The reactor was operated over a period of 30 d with a dilution rate of 0. 33 h-1, producing 1000-1200 U/mL of enzyme. The productivity of the re actor was in the range of 330-396 kU/h. A 20-fold increase in the prod uctivity with respect to batch fermentation with free cells was attain ed.