A. Quaas et al., ANTIBODIES AGAINST FUNGAL CONIDIA AND ANTIBIOTICS INHIBIT PHENYLALANINE AMMONIA-LYASE ACTIVATION IN CITRUS, Journal of plant physiology, 141(4), 1993, pp. 393-397
Antibodies raised against Trichoderma harzianum conidia prevented accu
mulation of Phenylalanine ammonia-lyase (PAL, E.C. 4.3.1.5.) in Citrus
. The observed effect of the antibodies is produced by a delay in the
formation of the fungal germination tube. An increase of PAL activity
of ca. 7 fold in C. limon and C sinensis seedlings, and of ca. 2 fold
in C. paradisii seedlings was found, as an hypersensitive response to
inoculation with T. harzianum conidia. This increase in activity was d
ue to <<de novo>> synthesis of the enzyme as demonstrated by the effec
t of protein-synthesis inhibitors and by immunotitration.