ORGAN-SPECIFIC REGULATION OF INTERFERON-GAMMA, INTERLEUKIN-2 AND INTERLEUKIN-2 RECEPTOR DURING MURINE INFECTION WITH TRYPANOSOMA-CRUZI

We have studied the expression of IFN-gamma, IL-2 and IL-2 receptor (I L-2R) at the mRNA and protein levels in spleen and lymph node cells fr om Trypanosoma cruzi-infected BALB/c mice. At 21 days post infection ( dpi) (peak of parasitaemia), spleen cells stimulated with Con A for 16 h showed a reduced IFN-gamma, IL-2 and IL-2R mRNA production compared with non-infected controls. Lymph node cells obtained at 4, 21 or 60 dpi produced similar amounts of IFN-gamma, IL-2 or IL-2R transcripts a fter mitogen stimulation as uninfected controls. Spleen cells obtained at 21 dpi showed a lower Con A proliferative response and IL-2R expre ssion compared with non-infected controls, while the proliferation and IL-2R expression of lymph node cells at 21 dpi was unaltered. Superna tants from 48 h Con A-stimulated spleen and lymph node cells from mice at 21 dpi had very low levels of IL-2 but contained significantly hig her levels of IFN-gamma compared with the supernatants of cells from n on-infected mice. The latter phenomenon correlates with an accelerated rate of IFN-gamma mRNA accumulation.