R. Asokan et al., PURIFICATION AND CHARACTERIZATION OF COLLAGENS FROM RAT FIBROSARCOMA INDUCED BY 3-METHYLCHOLANTHRENE, Molecular and cellular biochemistry, 121(2), 1993, pp. 99-107
The distribution of various collagen types was studied in rat fibrosar
coma. Collagens extracted from fibrosarcoma tissue were characterized
by the criteria of solubility in NaCl, SDS-PAGE, ion exchange chromato
graphy, CNBr peptide mapping and amino acid analysis. Fibrosarcoma was
found to produce excess amount of type V, type I trimer and type III
collagens; comparatively, type I collagen and total collagen content w
ere noticed to decrease in fibrosarcoma. We observe that the increase
in type V collagen content in fibrosarcoma might be due to the enhance
d transcription of type V collagen gene. Increased type I trimer colla
gen in fibrosarcoma might be attributed to the differential expression
of alpha1 (I) and alpha2 (I) gene and might also be due to the expres
sion of a different gene for type I trimer collagen.