ARYL MONOOXYGENASE - A DETOXIFYING ENZYME FROM CANDIDA-PULCHERRIMA MCMY2

Authors
DAY S
Citation
S. Day, ARYL MONOOXYGENASE - A DETOXIFYING ENZYME FROM CANDIDA-PULCHERRIMA MCMY2, Indian Journal of Biochemistry & Biophysics, 33(6), 1996, pp. 523-526
Citations number
15
Categorie Soggetti
Biophysics,Biology
Journal title
Indian Journal of Biochemistry & Biophysics → ACNP
ISSN journal
03011208
Volume
33
Issue
6
Year of publication
1996
Pages
523 - 526
Database
ISI
SICI code
0301-1208(1996)33:6<523:AM-ADE>2.0.ZU;2-F
Abstract
Characterization of partially purified aryl monooxygenase (1:14:14:1) from Candida pulcherrima MCMY2 was achieved using standard purificatio n protocol. The molecular weight of the enzyme was 110 kDa and contain ed 3 subunits with pI 5.7, 7.4 and 7.6. The activity seemed to be rela ted with pulcherrimin. The optimum pH and temperature for enzyme activ ity were 6.8 and 30 degrees C respectively. Activity was not substrate specific and Fe3+ FAD and NADH(+) enhanced the activity substantially .